ABSTRACT

Background: The gene known as methylenetetrahydrofolate reductase (MTHFR) is control of the production and methylation of deoxynucleic acids. Male infertility has been linked to the MTHFR polymorphism (C677T). Objective: A current study on male infertility was carried out to ascertain whether the MTHFR polymorphism (C677T) is connected to male infertility. The study included 30 male infertility patients aged between (26-58) years and 20 individual healthy control groups. The studied ages were distributed into three groups. The major group was (26 – 36) years, and the percentage was (40%). The second group was (37 – 47) years, and the rate was (26.67 %); the third group was (48 – 58) years, with a percentage (of 33.33%). The last group is the most prevalent among the three groups. Method: DNA was isolated, and Restriction Fragment Length Polymorphism–Polymerase Chain Reaction (RFLP-PCR) was done by using specific primers to the MTHFR gene. Results: the results indicated to the presence of mutations in 15 out of 30 patients after using restriction enzymes Hinf. Statistical analysis showed a correlation between male infertility and the emergence of MTHFR C677T gene mutation paralleled through the control group. Conclusion: When we compared the patient’s group and the healthy group, we established there is a correlation between MTHFR C677T gene polymorphism and the incidence of male infertility in our study population.