Joshua Allan Joseph¹, Uma Radhakrishnan², Sridhar Mutyala³, Krishnagouda Shankargouda Goudar³, Usha Parackal Thachappully Ayyappan¹, Amit Agarwal^3
1Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India
²Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.
³Department of Pharmacology and Toxicology, R and D Center, Natural Remedies, Bengaluru, Karnataka, India.
DOI: 10.4103/0976-9668.149119

ABSTRACT

Background: This study evaluated the antioxidant potential of a polyherbal formulation (Phytocee^™ ) in the rodent model. Materials and Methods: Four groups of rats (n = 6) were pretreated with Vitamin C (20 mg/kg) or Phytocee^™ (20, 100, and 200 mg/kg), respectively for 10 days. Oxidative stress in rat liver was induced by administration of carbon tetrachloride (CCl ₄ ) at 2 ml/kg as a single dose orally to all groups except the vehicle control group. After 24 h of administration of CCl ₄ , hepatic levels of malondialdehyde (MDA), serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic superoxide dismutase (SOD), catalase, and reduced glutathione (GSH) levels were evaluated.
Results: Phytocee^™ administered groups at all the dose levels significantly reduced the hepatic MDA, serum ALT and AST levels with a marked increase in hepatic SOD and catalase as compared with CCl ₄ treated group. Conclusion: The findings suggest that Phytocee^™ markedly reversed the effects of CCl ₄ induced oxidative stress and can be used as an antioxidant feed supplement.

Keywords: Antioxidant, carbon tetrachloride, malondialdehyde, oxidative stress, Phytocee^™.