Alka Grover1, SK Chakrabarti2, Neetu Jabalia1, AK Shrivastava1
1Amity Institute of Biotechnology, Amity University, Noida, UP-201301, India.
2Central Potato Research Institute, Shimla-171005, India.
ABSTRACT
Ralstonia solanacearum strain P082 a soil-borne bacterial plant pathogen causes brown rot disease in potato and mokodisease in banana. Normally, Moko disease-causing strains are not pathogenic to potato. Its further spread can only be controlled by use of effective detection methods. There are so many detection protocols available, ranging from culture based and immunodiagnostics to PCR amplification. PCR based methods are sensitive, specific and speedy.The aim of the present study was to developR. solanacearum strain P082 specific PCR primers for its detection. Primers were designed for conserved flanking regions ofshort tandem repeats present in its genome. Short Tandem Repeats (STRs) in R.solanacearum genome (Accession number CP002819 and CP002820)were searched using Tandem Repeat Finder software. A total of 159 and 114 STRs were found in chromosomal and megaplasmid DNA respectively. Sequence homology of these STRs analyzed using BLAST showed that out of total 273 STRs only few were found unique for R. solanacearumstrain P082. Correspondingly primers were synthesized for the flanking regions of these STRs. Read more…
