Comparison of the Effect of Combined Ginger Extract Nanosolutions on Growth Inhibition of Bacterial Isolates Causing Wound Infections

Abstract

Objectives: The aim of the study is to prepare safe, non-toxic nanomaterials and use them as alternatives in treating different types of bacteria, especially bacteria resistant to a large number of antibiotics commonly used in wound treatments in hospitals. Two nanocomposites were used, both of which were prepared using green synthesis. Methodology: Nickel nitrate with ginger and cobalt nitrate with Aloe vera were used as starting materials to prepare nanosolvents by the green synthesis method because it is a fast method for producing the nano solution. Additionally, many physical analyses and measurements were carried out without producing toxic waste and without requiring complex techniques. Results: The XRD showed that the films of the prepared solutions, deposited on glass by the drop casting method (nickel oxide with cobalt oxide), have a cubic phase and a polycrystalline structure. The morphological surface study of the nanocomposites by scanning electron microscopy showed that the particles were 127 nm in size. TEM (Transmission Electron Microscopy) revealed that the shape of the particles was spherical and semi-spherical and the average particle size was between 12-20 nm. The formation of NiO and CoO was confirmed in a high-purity phase by FT-IR spectra. The UV-Visible spectrum was used, where the band gap of the solution of the two nanomaterials (nickel oxide with cobalt oxide) was 2.75 eV. The effectiveness of the synthesized nanomaterial towards antibacterial activity was also determined. Here, good effectiveness was recorded against P. alcaligenes, Escherichia coli, Klebsiella pneumoniae, Staphylococcus epidermidis, Staphylococcus aureus, Enterobacter cloacae, P. Putida, Stenotrophomonas maltophilia, Acinetobacter baumannii, Pseudomonas aeruginosa. Conclusion: The synthesized nanoparticles can be used as a treatment against bacterial species that have multiple resistance to antibiotics in skin infections resulting from wounds.