ABSTRACT

Objectives: The primary objective of this study is to evaluate the antibacterial efficacy of dental bonding products including titanium oxide nanoparticles. Specifically, we aim to compare the antibacterial activity of these bonding agents with a control group consisting of bonding materials without the incorporation of nanoparticles. The target microorganisms for this investigation are Streptococcus sobrinus and Lactobacillus fermentum. The present study employed a standardised approach to describe the materials and methods used in the research. Materials and Methods: Two commercially available universal adhesives, namely Ambar universal (FGM, Brazil) and G-Premio Bond universal (Gc, America), were supplemented with a 4% mass fraction of a nano colloidal dispersion containing titanium dioxide. Initially, sterile discs composed of Whatman no. 1 filter paper were fabricated, with dimensions of 5 mm in diameter and 1 mm in thickness. Subsequently, a total volume of 100 microliters of each bonding agent was employed to fully saturate individual discs, which were subsequently exposed to a 40-second light curing process facilitated by an LED light source. Subsequently, the discs were positioned onto Mueller-Hinton agar. A total of 64 samples were extracted from the adhesives under investigation, and subsequently divided into four groups, each including 16 samples. Two sets of eight samples were collected from each group. Each group was further separated into two subgroups, with each subgroup consisting of eight samples. Subgroup A was designated to assess the antibacterial efficacy specifically targeting Streptococcus sobrinus. Subgroup B is designated for the purpose of evaluating the antibacterial efficacy specifically targeting Lactobacillus fermentum. Results: The study findings revealed that the dental bonding agent groups incorporating 4% exhibited considerably greater values for the inhibition zone compared to the control groups. The G-Premio bond universal groups, encompassing both control and integrated groups, exhibited notably larger inhibition zones compared to Ambar universal. Conclusions: The addition of titanium dioxide nanoparticles at a concentration of 4% to the universal bonding agents resulted in a notable enhancement of their antibacterial properties when compared to the bonding agents without the incorporation of nanoparticles.