ABSTRACT

Objective: The aim of the current study is to evaluate the histopathological morphology of transplanted tumors from the MCF-7 cell line into the nude mice after activation of their immune system, and to recognize the underlying pathways responsible for significant alterations. Methods: activation of immune system of natively immuno-deficient nude mice prior to transplant MCF-7 cell line of the human breast cancer, using means five mice for each control and experimental group. Subsequently, tumor samples were evaluated by histological examination to identify morphological aspect alteration. These morphological alterations were further evaluated to provide insights into the potential molecular pathways contributing to the histological changes, and possible outlook on improvement of cancer therapy and prognosis. Results: The histological examinations were done by expert pathologist and revealed a significant alteration in tumor morphology of MCF-7 cell line–transplanted into the activated immunity nude mice. The morphological alterations included a reduction of tumor growth, an increase in the inflammatory and immune cell infiltration with necrosis of tumor tissue (p value <0.05). The current experimental study illuminated potential molecular path was that could be contributing to the histo-morphological changes. Conclusion: The findings of the current study, highlight the importance of enhancing anti-tumor immune responses by active immunity, as a part of treatment of breast cancer. In addition, to potentiate the implications of immune-activating therapies. However, further both in vitro and in vivo studies are required to focus these findings and to explore the potential immunotherapeutic utility in treatment of patients with breast cancer.