Journal of Natural Science, Biology and Medicine, Vol 2, Issue 1, Jan-Jun, 2011 – Journal of Natural Science, Biology and Medicine

Intellectual property: Business of brains!

HS Arun KumarEditor in Chief, Journal of Natural Science, Biology and MedicineDOI: 10.4103/0976-9668.82303 It is our pleasure to bring in ...
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Management of intellectual property rights in India: An updated review

R Tiwari1, G Tiwari1, AK Rai1, Birendra Srivastawa21Department of Pharmaceutical Sciences, Pranveer Singh Institute of Technology, Kalpi Road, Bhauti, Kanpur ...
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Confocal images of DRG neuron cell bodies' double immunostained for PKC isoforms and TRPV1. PKC isoforms are indicated by the green fl uorescence of fl uorescein isothiocyanate (FITC) and TRPV1 is indicated by the red fl uorescence of Streptavidin Texas Red. Co-localization of PKC isoforms and TRPV1 is shown in overlay with yellow regions. Fluorescence intensity shown as traces represent localization of each PKC isoform (green trace) and TRPV1 (red trace) and is measured across an average of z-series cross-sections of the cell body of the neuron. Cytosolic and plasma membrane distribution of PKCI and PKCII are shown. Cytosolic distribution of PKC and PKC are shown. Method: The primary antibodies obtained from Santa Cruz. Their dilutions were rabbit anti-PKC-I (1/200), rabbit anti-PKC-II (1/200), and goat anti-VR1 (1/100). Secondary antibodies were goat anti-rabbit-FITC (1/100) (Sigma), rabbit anti-goat biotinylated species (1/100) (Sigma), and Streptavidin Texas Red (1/100) (Amersham Biosciences). All antibodies were diluted in Tris buffered saline (TBS) containing 50 mM Tris and 150 mM NaCl at pH 7.5. Controls included (i) replacement of the primary antibody with normal rabbit serum or (ii) goat serum or (iii) omission of primary antibody. No staining was apparent minus primary antibody or with normal serum controls. All experiments were replicated three times and at least two cultures were examined for each PKC isoform. Cultures were fi xed and permeabilized by incubating in cold (4°C) 3.5-4% paraformaldehyde in TBS for 20 min at room temperature (RT), washed three times in TBS, and blocked in blocking buffer 1 [1% bovine serum albumin (BSA), 1% fetal bovine serum (FBS) in TBS) for 1 h at RT. After washing in TBS, cultures to be double immunostained were incubated with the fi rst primary antibody at RT for 1 h, washed in TBS, and incubated with the fi rst secondary antibody (anti-rabbit FITC) for 1 h at RT; it was further incubated with the second primary antibody (goat anti-TRPV1) at 4°C overnight. After washing in TBS, the cultures were incubated in blocking buffer 2 (10% BSA in TBS) for 10 minutes at RT, followed by TBS wash and a two-step incubation with second secondary antibody. The fi rst step involved incubation of the cultures with rabbit anti-goat biotinylated species for 1 h at RT. In the second step, the cultures were washed as previously and incubated with Streptavidin Texas Red for 1 h at RT. After washing, coverslips were inverted on to microscope slides in a 25-L fl uorescence anti-fade solution (Vector Laboratories) and sealed with clear nail polish. Immunostained cultures were visualized by a confocal laser scanning microscope (CLSM) using Leica TCS SP2 System fi tted with PL APO 100x/ 1.40-0.7 OIL objective). Sequential scans (FITC and Texas Red) were collected and merged and quantifi ed using Leica TCS SP2 Software ver. 3.0. Fluorescence intensities of FITC and Texas Red were measured across the merged images of neurons as a function of distribution of the specifi c PKC isoforms and TRPV1, respectively, from the cytosol to the plasma membrane.

Protein kinase C modulation of thermo-sensitive transient receptor potential channels: Implications for pain signaling

Sravan Mandadi1, Patricia J Armati2, Basil D Roufogalis31Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada; Faculty of Pharmacy, The ...
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The subgranular zone (SGZ) of the dentate gyrus shelters the astrocyte like stem cells (1), which generate the transit amplifying progenitor cells (2). These transit amplifying cells mature into neuroblasts (3), which migrate (4) in the vicinity into the granule cell layer (GCL) and differentiate into granule neurons (5). These newly generated granule neurons show initial signs of functional interconnections by extending axonal projections along the mossy fi ber towards the CA3, and advancing their dendritic branches into the molecular layer (ML). Once these cells mature, they receive glutamatergic (Glu) inputs from the entorhinal cortex (EC), GABAergic inputs from the interneurons and provide glutamatergic inputs to the CA3 neurons.

Regulation of adult neurogenesis in the hippocampus by stress, acetylcholine and dopamine

J Veena1, BS Shankaranarayana Rao2, BN Srikumar31Laboratoire Psynugen, Université Bordeaux 2, Bordeaux, France.2Department of Neurophysiology, National Institute of Mental Health ...
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Therapeutics of stem cells in periodontal regeneration

Rajiv Saini1, Santosh Saini2, Sugandha Sharma31Department of Periodontology and Oral Implantology, Rural Dental College - Loni, Maharashtra, India.2Department of Microbiology, ...
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Immunity over inability: The spontaneous regression of cancer

Thomas JessyDepartment of Oral Medicine and Radiology, Dr. Syamala Reddy Dental College and Hospital, Marathahalli, Bangalore, Karnataka, India.DOI: 10.4103/0976-9668.82318 ABSTRACT ...
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Periodontitis: A risk for delivery of premature labor and low birth weight infants

Rajiv Saini1, Santosh Saini2, Sugandha R Saini31Department of Periodontology, Rural Dental College-Loni, Pravara Institute of Medical Sciences, Loni, Ahmednagar, Maharashtra, ...
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Science behind human saliva

Manjul TiwariDepartment of Oral Pathology and Microbiology, School of Dental Sciences, Sharda University, Greater Noida, Uttar Pradesh, India.DOI: 10.4103/0976-9668.82322 ABSTRACT ...
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General aspect of Maytenus heterophylla in the natural habitat. Reproduction size: column width

Maytenus heterophylla and Maytenus senegalensis, two traditional herbal medicines

G da Silva, R Serrano, O SilvaiMed.UL, Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-019 Lisboa, Portugal.DOI: ...
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Ozone therapy: A clinical review

AM Elvis, JS EktaViveknand Education Society's, College of Pharmacy, Mumbai, India.DOI: 10.4103/0976-9668.82319 ABSTRACT Ozone (O 3 ) gas discovered in ...
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Epidemiology and symptoms of red eyes in patients from Northern Iran

Biofilm: A dental microbial infection

Rajiv Saini1, Santosh Saini2, Sugandha Sharma31Department of Periodontology and Oral Implantology, Rural Dental College, Maharashtra, India.2Department of Microbiology, Rural Dental ...
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Phytochemical investigation and evaluation of antinociceptive activity of ethanolic extract of Dalbergia sissoo (Roxb.) bark

Mohammad Asif, Arun KumarDepartment of Pharmacy, Faculty of Pharmacy, GRD (PG) IMT Rajpur, Dehradun - 248 901, Uttarakhand, IndiaDOI: 10.4103/0976-9668.82315 ...
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Effect of SNAP, L-NAME, and SNAP and L-NAME on fasting and postprandial blood glucose concentrations

The effect of nitric oxide inhibitors and Snitroso-Nacetylpenicillamine on glucose concentration in an animal model

Sophia Bryan1, Ruby Alexander-Lindo1, Tara Dasgupta2, Donovan McGrowder31Department of Basic Medical Sciences, Faculty of Medical Sciences, The University of the ...
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A scientometric analysis of Indian research output in medicine during 1999-2008

BM Gupta1, Adarsh Bala21National Institute of Science, Technology and Development Studies, New Delhi - 110 012, India.2Government Medical College and ...
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Obesity in Kolkata children: Magnitude in relationship to hypertension

Partha Chakraborty1, Subrata Dey2, Ranabir Pal3, Sumit Kar3, Forhad Akhtar Zaman3, Shrayan Pal41Aditya Hospital, Kolkata, India.2Department of Pediatrics, Apollo Gleneagles ...
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Table 3: Serum MDA levels of diabetics in different ethnic groups in Sikkim

Serum malondialdehyde level: Surrogate stress marker in the Sikkimese diabetics

Yazum Bhutia1, Amrita Ghosh1, Mingma L Sherpa1, Ranabir Pal2, Pradip Kumar Mohanta31Department of Biochemistry, Sikkim Manipal Institute of Medical Sciences ...
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Fine needle aspiration cytology as the primary diagnostic tool in thyroid enlargement

Arup Sengupta1, Ranabir Pal2, Sumit Kar2, Forhad Akhtar Zaman2, Subhabrata Sengupta3, Shrayan Pal21Department of Otorhinolaryngology, M.G.M. Medical College, Kisanganj, Bihar, ...
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Table 1: Clinico-social cofactors of the study participants

Evaluation of Ondansetron-induced QT interval prolongation in the prophylaxis of postoperative emesis

Sampa Dutta Gupta1, Ranabir Pal2, Aniruddha Sarkar1, Sudakshina Mukherjee1, Koel Mitra1, Suddhadeb Roy1, Debabrata Sarbapalli3, Kanak Kanti Kundu1, Forhad Akhtar ...
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small swelling in relation to the mandibular canine and the mandibular right fi rst premolar

Heterotopic salivary gland tissue: A report of two cases

S Gheena, T Chandrasekhar, Pratibha RamaniDepartment of Oral and Maxillofacial Pathology, Faculty of Dental Sciences, Sri Ramachandra University, Chennai,Saveetha Dental ...
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Klestadt’s cyst

Reet Kamal1, Parveen Dahiya2, Sangeeta Palaskar31Department of Oral and Maxillofacial Pathology, HIDS, Paonta Sahib, (H.P.), India2Department of Periodontics, HIDS, Paonta ...
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Biomedical Research – From Ideation to Publication

Pitchai BalakumarDepartment of Pharmacology, Institute of Pharmacy, Rajendra Institute of Technology and Sciences (RITS), Sirsa-125055, Haryana, India. Research is an ...
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