KP Sharmila1, B Satheesh Kumar Bhandary1, Ronald Fernandes2, N Suchetha Kumari3, Vadisha S Bhat4, K Jayaram Shetty5, Jerish M Jose5, Alex John Peter6
1Central Research Laboratory, K.S Hegde Medical Academy, Nitte (Deemed to be University), Mangalore, Karnataka, India.
2Department of Pharmaceutical Chemistry, NGSM Institute of Pharma Sciences, Nitte (Deemed to be University), Mangalore, Karnataka, India.
3Department of Biochemistry, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Mangalore, Karnataka, India.
4Department of ENT, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Mangalore, Karnataka, India.
5Department of Oncology, Nitte Leela Narayan Shetty Memorial Cancer Institute, Mangalore, Karnataka, India.
6Department of Radiation Oncology, Apollo CBCC, Ahmedabad, Gujarat, India.
Background: Radiotherapy is an important and the most common treatment modality for human cancers. Cancer radiotherapy is associated with unadorned side effects that results from normal tissue damage which is a major subject of concern. Radiation induces damage to living cells due generation of aqueous-free radicals. Therefore, there is a crucial need for the protection of normal cells surrounding the tumor from radiation injury; and hence, the identification of radiation-protecting agents is a chief goal for basic radiation biologists and oncologists. Aim: The aim of this present study was to assess the radioprotective potential of Asparagus racemosus root ethanolic extract (ARE), and isoprinosine (IPR) against electron beam radiation (EBR)-induced immunosuppression and oxidative stress in Swiss Albino mice. Materials and Methods: Swiss albino mice were used for the assessment of the radioprotective potential of ARE and IPR against EBR-induced immunosuppression and oxidative stress. Cytokine estimations, namely, interleukin-2, interferon-gamma, and tumor necrosis factor-alpha were performed in the liver homogenate using ELISA kits, and bone marrow cellularity was determined in the experimental animals. Results: The results of the present study demonstrated the radioprotective and immunostimulatory efficacy of ARE and IPR against EBR-induced cytokine and bone marrow cellularity alterations. Conclusion: The findings of our study demonstrate the potential of ARE and IPR in mitigating radiation-induced mortality by offering protection to mice against lethal dose of whole body EBR. The present study also demonstrates that ARE and IPR exert its radioprotection against EBR induced immunosuppression by regulating cytokines.
Keywords: Asparagus racemosus, Electron beam radiation, Immunosupression, Isoprinosine, Oxidative stress.