P Ramalingam1, Y Padmanabha Reddy2, K Vinod Kumar2, Babu Rao Chandu3, K Rajendran1
1 College of Pharmaceutical Sciences, Gulf Medical University, Ajman, UAE
2 Division of Pharmaceutical Analysis and Quality Assurance, Raghavendra Institute of Pharmaceutical Education and Research, Anantapur, India
3 Faculty of Pharmacy, Al-Zawiya University, Zawiya, Lybia
Introduction: The IR absorption patterns (in cm−1 ) provide the basis to distinguish among the constituents and to separately quantify as well as qualify them and they possess many advantages such as very small sample volume requirement, good precision over entire physiological range, avoid of costly disposables, wealth of information from a single spectral measurement. The efficacy of anti-diabetic drug metformin hydrochloride as used to treat diabetic-induced Wistar rats and their sera were analyzed by FT-IR (ATR) in absorption mode. Materials and Methods: The present work was attempted in the study of normal and antidiabetic regimen-treated rat blood samples using FTIR spectroscopy by the attenuated total refl ectance (ATR) sampling technique. The biomolecule characteristics were measured as intensity ratio parameter (IRP) values and interpreted. Results: To quantify the results three IRPs such as R1, R2 and R3 were calculated, respectively, for lipid, protein, and glucose. The glucose IRP value R3 showed, 0.3802, 0.3304, and 0.2847, respectively, for diseased, metformin-treated, and normal rats. Conclusion: The IRP values for glucose are compared to the glucose level obtained by using a glucometer. This study can be conveniently used in diagnostic procedures, patient compliance assessment, and efﬁcacy evaluation of metformin hydrochlorides.
Keywords: Fourier transform, Infrared spectroscopy, antidiabetic, metformin, blood glucose