Keesoo Lee1, Megan L Eisterhold1, Fabio Rindi2, Swaminathan Palanisami1, Paul K Nam3
1 Department of Life and Physical Sciences, Center for Bioenergy, Lincoln University of Missouri, Jefferson city, MO 65101, Italy
2 Department of Life Sciences and Environment, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy
3 Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA

DOI: 10.4103/0976-9668.136178


Native species of microalgae were isolated from natural water bodies in the Midwestern United States of America and were screened for the ultimate goal of mass cultivation in Missouri and the surrounding states, and for their potential as biomass and biodiesel sources. A number of different nutrient media recipes were utilized to isolate the maximum number of colonies from each field samples. These nutrient recipes were modified in order to optimize the isolation and growth dynamics of specific colonies. All of the isolates were categorized based on the morphological appearance of the culture and the microscopic cellular appearance of the isolated colonies. Isolates included many common green microalgae and cyanobacteria. Lipid content was determined for selected strains that demonstrated relatively quick growth. Scenedesmus sp. that demonstrated the high growth rate, resistance to invasion, and contained sufficient amounts of lipid was investigated for its potential as a sustainable biomass and biodiesel feedstocks.

Keywords: Biodiesel, isolation, lipids, microalgal biomass, Midwestern USA

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