Asif Elahi1, Javed Ahmad2, Mohd. Nauman2, Humayra Bashir2, Farah Khan1, Mohd. Irfan Qureshi2
1Department of Biochemistry, Jamia Hamdard, New Delhi-110062, India.
2Department of Biotechnology, Jamia Millia Islamia, New Delhi-110025, India.
Fish are largely being used for the assessment of the quality of aquatic environment and as such can serve as bioindicators of environmental pollution. Heavy metals accumulated in the tissues of fish may catalyze reactions that generate reactive oxygen species (ROS) which may lead to environmental oxidative stress. Defensive mechanisms to counteract the impact of ROS are found in many species including fish. Hence comparative protein profile and Antioxidant analysis were performed in the liver tissue of Walking Cat Fish (Clarias batrachus). Level of thiobarbituric acid reactive substances (TBARS), activity of ascorbate peroxides (APX), superoxide dismutase (SOD) catalase (CAT) and contents of ascorbate and glutathione were estimated spectro-photometrically. The statistical significance of difference between control and treated were determined by student’s t-test using graphpad instat software. Statistical significance was defined as a P value <0.05. SDS-PAGE profile of liver protein was resolved obtained from C. batrachus fish collected from pond (control) and the ones collected from the banks of Yamuna River, Delhi. Results obtained showed elevated level of TBARS (33.33%), Ascorbate (25%) and Glutathione (55%) and activity of SOD (87%) and CAT (54%) and APX (19%) as compared to control. Protein profile showed changes in expression pattern over control. Results indicate that pollutants present in the Yamuna water like heavy metals could elevate the oxidative stress which in turn further elevates the activities of non-enzymatic and enzymatic antioxidants to overcome the oxidative induced by ROS. Changes in protein profile and identification of specific protein band(s) may serve as potential markers for oxidative stress induced by environmental pollution. Read more…