HM Kantharaj, SK Ashwath, S.M.H Qadri
Bioinformatics Centre, Central Sericultural Research and Training Institute, Mysore, India.

ABSTRACT

The proteomic studies in general and prediction and structural elucidation of protein, in particular adapt an in silico approach. With the advent of functional genomics and the availability of comprehensive publicly accessible Bombyx mori Nuclearpolyhedrovirus (BmNPV) sequence database, the present study has been carried out in silico to predict the structure and function of a P74 protein of BmNPV. The analysis in the present study showed conserved domain characteristics of Baculovirus P74 super family characteristics. The modelled protein exhibited a maximum number of random coils (44.19%) with alpha helix (39.69%) and extended strands (16.12%) as secondary structural elements. Since the computed instability index of the protein is 39.65, the protein was found to be stable.. It is reported that the hydrophobic C terminus of P74 plays an important role in protein localization and possibly in transmembrane anchoring of the virus into the host cell. So for the secondary structure prediction we took the proteins which are very important in terms of infecting Bombyx mori cells both in vitro and in vivo. Read More …