Sukumar Sinha, Vikas Parashar, K Swamy Reddy, Mohammad A Khan, S. K. Manirul Haque
Department of Analytical Research Lab, Glenmark Generics Ltd, Glenmark Research Centre, Plot A – 607, TTC Industrial Area, MIDC, Mahape, Navi Mumbai, Maharashtra, India.
DOI: 10.4103/0976-9668.117018

ABSTRACT

A simple, sensitive method for the determination of meta-chlorobenzoic acid in bupropion hydrochloride is described. Chromatographic separation of m-chlorobenzoic acid is achieved using a mobile phase consisting of n-hexane and ethanol (1000:50, v/v) at a flow rate of 1.0 ml/min on a Chiralpak ADH (250 × 4.6 mm). Absorbance is monitored at 235 nm. The method is linear for m-chlorobenzoic acid over concentration range of LOQ, 5.0 μg/ml to 15.0 μg/ml for m-chlorobenzoic acid with correlation coefficient greater than 0.99. This method is more selective and accurate than United States Pharmacopoeia method for the determination of m-chlorobenzoic acid in bupropion hydrochloride. Read more…

Keywords: 1-(3-Chlorophenyl)-1,2-propanedione, meta-chlorobenzoic acid, bupropion Hcl ,HPLC method , method validation.