Tahereh Shahmoradi1, Hamid Sepehry2, Manuchehr Ashrafpour3
1Department of Physiology, Faculty of Medicine, International Branch of Shahid Behshti University of Medical Sciences, Shemiranat, Tehran, Iran.
2Neuroscience Research Center, Golestan University of Medical Sciences, Gorgan, Golestan, Iran.
3Department of Physiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Mazandaran, Iran.
Background: Amphotericin B (AmB) is a polyene antibiotic frequently applied in the treatment of systemic fungal infections in spite of its secondary effects. The pH plays a crucial role in modulating biophysical features of ion channels in the bilayer lipid membranes. Aim: In this study, the role of pH in the regulation of AmB channel was assessed by single channel recording of ion channel incorporated in the artificial membrane. Materials and Methods: Bilayer lipid membrane was formed by phosphatidylcholine in a 350 ΅m diameter aperture between two chambers, cis and trans contained 200/50 mMKCl solutions, respectively; then AmB was incorporated into the bilayer lipid membrane. Single channel recordings were used to indicate the effects of pH changes on AmB channels activity. The records were analyzed by Clamp fit 10 software. Results: A kinetic analysis of single channel currents indicated a cation ion channel with 500 pS conductance and voltage-dependence of the open probability of the AmB channel (Po ). A reduction of cis pH to 6 decreased Po and conductance. This effect was also voltage-dependent, being greater at a more positive above −40. The pH changes in the range of 6-8 had no effect on the reversal potential and ion selectivity. Conclusion: Our data indicated that extracellular acidity can reduce AmB activity.
Keywords: Amphotericin B, bilayer lipid membrane, phosphatidylcholine, single channel recording.