Anjani Saxena, Shantanu Tamuly, MK Saxena
Animal Biotechnology Center, Department of Veterinary Physiology &Biochemistry College of Veterinary Sciences and Animal Husbandary, G.B.P.U.A&T, Pantnagar-263145, Uttrakhand, India.
Typhoid is one of the most important diseases of human beings caused by Salmonella Typhi. There are many vaccine reported against Salmonella typhi. Presently available vaccines have several limitations such as short term immunity, high cost and allergic reaction. Several approaches such as subunit vaccines, Vi polysaccharide, mutant vaccines and r-DNA vaccines have been tested in last few years among which r-DNA vaccines (targeted Omp) have shown some promising potential. In the present study Omp 28 gene of S.Typhi sequence was analyzed for B cell, T cell epitope and motif analysis. On Prosite analysis of Protein shown two motifs i.e. anaphylatoxin domain signature motif at position 219-252 and other one was iron sulphur binding region signature motif at position 36-44. On Pfam and Swiss prot model analysis no similar protein could be identified in the database. On epitope analysis total six major B cell epitopes were observed which can provoke humoral immunity but as Salmonella is a intracellular pathogen T cell mediated immunity is more important. A critical step in developing immune response against pathogens is the recognition of antigenic peptides presented by MHC Class I and II molecule. On T cell epitope mapping several major epitopes has been found in case of MHC class I and MHC class II. It indicates that Omp 28 can provoke cell mediated as well as humoral immunity and can be proven a promising candidates of Salmonella typhi. Read More …