Munazzah Tasleem, Seemi Kalam, Faizan Ahmad, Md. Imtaiyaz Hassan
Centre for Interdisciplinary Research in Basic Sciences, Jamia Nagar, New Delhi 110025, India.

ABSTRACT

Candida dubliniensis genome contains 8 chromosomes, 6095 gene sequences, 5860 protein coding sequences. Out of these 5860 protein coding sequences 43 hypothetical proteins (HPs). We used all 43 HPs for functional annotation. Assignment of function to the HPs started with finding sequence similarity using FASTA & PSI-BLAST, then to find out motifs and structural domains using PROSITE, PRINT, InterPro, ProDom, Pfam, SMART and BLOCKS. Three-dimensional structure of these HPs was modeled by using SWISS-MODEL. We have observed structurally conserved regions in the modeled structure using DaliLite, TOPS, SSM, VAST, GRATH, SSAP, MATRAS and LOCK2, to help in functional evaluation. Predicted functions of HPs of C. dubliniensis are, synthesis, membrane, cell division, transcription, group transfer, kinase, binding, structure, DNA synthesis, hydrolysis and transport. Sequencing of multiple genomes of any organism and concomitant development of structural genomics create an opportunity that aimed for producing a complete catalog of biochemical activities, biological functions and the responsible genes for the virulence and pathogenesis. Functional annotations of HPs are required to predict the possible drug targets. Our function assignment to conserved hypothetical proteins from C. dubliniensis provides a potential to achieve a precise function in a cost effective manner. Read More …